Research indicates that individuals with BP-I remain symptomatic for nearly half of their illness course, with depressive symptoms being the most frequent and persistent, significantly outweighing manic/hypomanic and mixed episodes (Nierenberg et al., 2023)
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Briefly, a 24-well culture plate was coated with 200 l (9.1 mg/ml)/well Matrigel prepared from Engelbreth-Holm-Swarm mouse tumors (Corning, MA, USA) and allowed to stand for 30 min at 37 C to form a gel layer
Half an hour before the end of the incubation with the indicated stimulus, Hoechst was added to each well of 24-well plates at a final concentration of 1 M
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