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Incubations of the cells were carried out in microplates at a density of 1.5 10 4 cells per well in HBSS buffer (Invitrogen) with 20 mM HEPES (pH 7.4) and 500 M IBMX
Circulating tumor cells isolation, counting, and IF staining Circulating tumor cells (CTCs) were isolated using EPCAM + magnetic beads (130-105-958, Miltenyi Biotec, Bergisch Gladbach, Germany)
A separate study (111) found liraglutide alleviated sensory deficit after focal cerebral ischemic stroke, which may be related to the improvement of A3-16 deposition as well as secondary damage in the ipsilateral thalamus
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