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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development
The lipid dependence of the fluidizing effects of anesthetics was also observed in a study on dibucaine in various mixtures of POPC:POPE:CL, showing that the diffusion coefficient varies with lipid composition with an obvious dependence on cardiolipin [342]
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