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By treating metabolically active adipocytes and hepatocytes with 5-amino-1-methylquinolinium, researchers can precisely inhibit intracellular NNMT activity, monitor changes in the nicotinamide pathway and NAD+ levels, and analyze effects on energy metabolism

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Lysates were clarified by centrifugation at 8000 g for 5 min at 4 C, and the supernatants were collected

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