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H.BingleL

Segmentation of the cells was performed based on the image resulting from the Hoechst channel using first the IdentifyPrimaryObjects module for segmentation of nuclei based on a global three-class Otsu threshold method using intensity to distinguish and draw dividing lines between clumped objects, followed by the IdentifySecondaryObjects module to segment cells using the Distance-N function with a fixed maximum distance from the nucleus to cell border