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Inject 2mg subcutaneously three times weekly (Monday/Wednesday/Friday)
The culture medium was then removed after 24 hours incubation
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Previous reports indicate, that sea urchins in general, have a high cysteine content, which serves various important roles including: (1) reinforcing intermolecular disulphide bonds, for maintaining protein structure 55 , (2) binding sites for heavy metals, supporting detoxification processes 56 , (3) regulating osmotic pressure by enhancing taurine synthesis 27 and (4) maintaining the redox state and supports antioxidant functions 57,58,59
As shown in Figure 5B, compared with the model group, the cell viability of PC12 cells increased accordingly as the daphnetin concentration increased, and the daphnetin concentration of 15 g/mL had the best protective effect
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