We assessed the relationships between the GLP-1 RAs users and the control group concerning primary and secondary outcomes using the Cox proportional hazards model, which allowed us to calculate adjusted hazard ratios (aHRs) 51
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Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h
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There will be an estimated 1-2 pounds of weight loss per week
