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For perfusion of the small intestine, a catheter was inserted into the superior mesenteric artery, and the intestine was vascularly perfused with heated (37 C), oxygenated (95% O 2 and 5% CO 2 ) perfusion buffer at a constant flow rate of 7.5 ml/min using a single pass perfusion system (UP100, Hugo Sachs Harvard Apparatus, Germany)
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