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For reproducers in period of rest

Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions

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Intensive care with continuous veno-venous hemodiafiltration was required, resulting in full recovery

This shows how temperature directly affects sensitivity to chemicals and thereby the development of the Green Frog by making their habitat more dangerous