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After drug release, a Perkin-Elmer 200 series HPLC system with a Waters 2487 UV detector and a Bioscan Flow-Count detector equipped with an analytical C-18 HPLC column (XTerra 5 m, 150 4.6 mm, Waters) was used for analyzing the released Ex4

Of note, the dopamine neurons in the ARH also regulate pituitary hormones (71), which could be linked to the GLP-1 stimulatory release of pituitary hormones, such as vasopressin and corticotropin-releasing hormone (72)

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