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Expression and purification of recombinant APEX2 APEX2 was expressed from a plasmid (72558 pTRC-APEX2, Addgene) in Escherichia coli BL21(DE3) (EC0114, Thermo Fisher Scientific), as described previously 23
Thats why the suggested dose is to build up slowly over five days, after which the digestive issues usually pass
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