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Peptide therapy does not require anesthesia because the injections are not painful
Standard subcutaneous injection technique applies: pinch a fold of skin, insert the needle at 45-90 degrees, inject slowly, withdraw and apply gentle pressure
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Additionally, various molecular techniques, including Western blotting, reverse transcription, quantitative PCR, immunofluorescence, and immunohistochemistry, were utilized to examine the expression levels of angiogenesis-related factors and the Notch/NF-B pathways-related factors
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