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Peptides were desalted using StageTips (Thermo Fisher Scientific), dissolved in solvent A (0.1% (v/v) FA), and resolved on a 75 m 50 cm, 2 m particle size, EASY-Spray PepMap RSLC C18 column (Thermo Fisher Scientific) heated to 45 C, and coupled to a 75 m 2 cm, 3 m particle size, Acclaim PepMap 100 pre-column (Thermo Fisher Scientific) run on an EASY-nLC 1000 UHPLC (Thermo Fisher Scientific)
In contrast, members who stopped taking their medication before 24 weeks lost an average of only 7.4% of their body weight, a 64% relative difference
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Some common symptoms include hot flashes, night sweats, mood swings, sleep disturbances, vaginal dryness, and weight gain
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