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For typical use patterns, bacteriostatic water's 30-day reconstituted shelf life better matches consumption timelines

Commonly used soft-tissue protocols include 250 mcg once daily, 500 mcg once daily, or 250 mcg twice daily

The supplemented group showed decreased respiratory quotient, increased fat oxidation percentage, reduced insulin levels, improved insulin sensitivity (HOMA index), reduced waist circumference, and increased resting energy expenditure compared to placebo

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Agarose gels were all 1.5% w/v agarose stained with ethidium bromide (Fischer) and imaged on BioRad GelDoc imager