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10.1038/s41467-018-05336-9 169 ZengZ.YuR.ZuoF.ZhangB.PengD.MaH.et al (2016)

Positive clones were selected for by puromycin treatment, single-cell sorting, cell expansion, and confirmation by gene amplification and sequencing for correct gene cassette insertion, and immunoblotting for expression of the Cas9 protein after doxycycline treatment

The epidemiological data for a vagal route is equivocal

The glutathione building blocks in whey are very fragile, but techniques to extract these valuable proteins have been developed

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