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The precipitate was washed with the same weight of 10% TCA three times, and the protein-bound form of glutathione was extracted with the same weight of 10% TCA-4% 2-mercaptoethanol as described above
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Additionally, the combined treatment also affected HEB cells, although the tolerance of HEB cells to the drugs was higher compared to glioblastoma cell lines (Fig
10.1097/aog.0000000000004538 63 WarkusE
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