When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)
Dose-escalation decisions in both phases are evaluated by a Safety Review Committee (SRC) , which assesses clinical, pharmacokinetic and safety data according to predefined stopping rules established in the study protocol
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However, discuss any concerns with a doctor to prevent unwanted health issues