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1.3 In vitro Tirzepatides effects In vivo and in vitro preclinical studies have demonstrated that co-administration of GIP and GLP1 promotes insulin sensitivity, leading to better blood glucose level control, more sustained reduction in food intake and consequently better WL compared to the infusion of a single agent (35, 41, 46), also exerts an additive effect on metabolism of cyclic adenosine monophosphate (cAMP), thus increasing its levels and consequently, potentiating insulin secretion glucose-dependent

Rats were bred in-house at the Animal Pharmacology Facility, School of Medicine, Zagreb, Croatia

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