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Notably, cumulative food intake for 24 h tended to be suppressed without rebound for the following 2448 h period (Fig
The timing depends on individual recovery and digestive stability
Then cells were suspended in Stimulation Buffer (provided with the assay kit), added to the plate (120,000 cells/50 l/well), shaken 5 minutes at room temperature (RT) and then incubated 25 minutes at RT in the absence or presence of mAb 3F52
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