It is one of the best-known short peptide references in discussions around gastric peptide biology, tissue-signalling models, nitric-oxide pathways, and extracellular matrix research
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Kranti were collected from agricultural farm of the university, sterilized with 0.1% sodium hypochlorite solution following repeated washing and germinated on Petri plates in seed germinator (BTR Kolkata, LAB-X)
Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0
Every batch is analysed in-house by HPLC and mass spectrometry and ships with a Certificate of Analysis