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During the following days, cell proliferation was assessed by real-time imaging of confluence using an IncuCyte Zoom system (Essen BioScience) with 6 technical replicates per condition
Western blot analysis data presented that compared with the cells without treatment, the extent of p38 MAPK phosphorylation was increased in cells treated with CSE, and compared to CSE cells treated with PBS, it was reduced in CSE cells treated with NAC (Fig
reassess for any new symptoms (edema, pericardial effusion signs, unexplained fatigue) Ongoing: Annual metabolic panel if both agents are continued long-term
Foods with glutathione are great asparagus, avocados, spinach
Detection of protein carbonylation Levels of carbonylated proteins were determined with the OxyBlot protein oxidation detection kit following the manufacturers protocol (Millipore, Billerica, MA, USA)
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