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Burgmaier M, Liberman A, Mollmann J, Kahles F, Reith S, Lebherz C, et al

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Roles of NADPH in Reductive Biosynthesis NADPH in Fatty Acid Synthesis During the de novo synthesis of palmitic acid, fatty acid synthase (FAS) utilizes NADPH for two of the reactions of each cycle of acetyl-CoA addition

Here we determined the rate constants for the individual redox reactions between the two redox-sensitive cysteinyl residues of roGFP2 and the active-site cysteinyl residues of the model class I glutaredoxin PfGrx from the malaria parasite Plasmodium falciparum using stopped-flow kinetic measurements in combination with redox mobility shift assays