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This resulted in a dramatic reduction of the high-energy barriers previously obtained with DFT or QM/MM methods, often to zero, enabling the study of vitamin B12 cofactors in in vivo processes

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pyrenoidosa , chlorophyll a content decreased from 2.69 mg g -1 in the control to 2.17 mg g -1 at 300 g L -1 Pb and 0.92 mg g -1 at 500 g L -1 Pb, representing declines of 19.3% and 65.8%, respectively, compared with the control