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Demonstrated positive response defined as 5% reduction from baseline body weight (adults) or baseline BMI (pediatrics), or maintenance of weight loss with continued participation in a weight management program

Slides were incubated in a humidified chamber for 1 hr at RT in secondary antibodies: donkey anti-rabbit Alexa Fluor 647 (1:1000, Invitrogen, #A31573), goat anti-mouse Alexa Fluor 488 (1:1000, Invitrogen, #A11001), goat anti-rabbit Alexa Fluor 594 (1:100, Invitrogen, #A37117), and goat anti-rabbit Alexa Fluor 488 (1:400, Invitrogen, #A32766) prepared in their respective blocking buffer

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This can simplify liposuction and even reduce the volume of fat that needs to be extracted

discussion 1862-1853