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Timing your injection on a consistent day each week and pairing the protocol with adequate protein intake (80 to 100g daily), proper hydration (64 to 80 ounces of water), and small frequent meals dramatically improves both results and tolerability

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For immunochemistry staining, the sections were treated with 3% hydrogen peroxide for 10 min to block endogenous peroxidase activity, and incubated in buffered normal horse serum to prevent the non-specific binding of antibodies