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A potential anti-inflammatory novel target is HCK kinase, which plays a role in inflammatory processes through NLRP3 inflammasome activation, which promotes the maturation and release of pro-inflammatory cytokines, linking it to the development of inflammatory conditions like type 2 diabetes, atherosclerosis, and Muckle-Wells syndrome (Kong et al., 2020)

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Ligands were added immediately prior to reading on a Mithras LB 940 multimode microplate reader (emission filters at 400 nm and 515 nm)