Draw 1ml of the Lipotropic (labeled as MIC/B12) into the syringe
Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue
Although food can be the source of vitamin b12, there are certain conditions that do not favor the cobalamin absorption or make it excretes out of the body in large amount
10.1186/s13195-016-0207-9 Alzheimers Res
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