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Indeed, undifferentiated TC cells express low levels of sodium/iodine-symporter (NIS), a protein membrane crucial for iodine uptake by thyrocites and consequently essential for the efficacy of RAI, the most effective therapy for TC in case of not complete surgical removal
Data were further processed with QuantStudio Real-Time PCR Software v1.3
Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer
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Each method has its unique set of benefits, driven by factors such as bioavailability, convenience, cost, and personal health goals
