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Glutathione's Critical Role in Liver Function The liver handles Phase I and Phase II detoxification , with glutathione playing an indispensable role in both pathways

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For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)

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Miller CM et al