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Likewise, transcriptomic studies have shown that many multifactorial stress-responsive genes are absent from corresponding single-stress datasets, highlighting extensive regulatory reprogramming under combined environmental conditions (Nawaz et al., 2023)

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This multistep procedure performs the preliminary hydrogen atoms addition employing the pdb2pqr 46 and PROPKA3 47 software combination through the systemPrepare HTMD implementation 48 , considering a simulated pH of 7.4

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