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These are often dose-dependent and can be minimized by starting with a lower dose (e.g., 500 mg) and taking it with food
PMCID: PMC7120747
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Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis
Sleep architecture itself exhibits a cyclical pattern, typically oscillating between NREM and REM phases multiple times throughout the duration of total sleep
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