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The Calu3 cell line is the most widely used model to reproduce the upper respiratory epithelium, as it is able to recapitulate differences in mucus layer thickness and cytokine production when grown under liquid-liquid interface (LLI) or air-liquid interface (ALI) conditions

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During this process, the target protein is attached to a solid-phase carrier (e.g., avidin-streptavidin tags, GST tags, His tags), where it interacts with other proteins in the solution

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