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AUC was significantly increased during cocaine seeking compared to baseline in VTA GABA neurons of rats pretreated with exendin-4, indicating that cocaine-seeking behavior evokes transient increases in VTA GABA neuron activity in exendin-4-treated rats (Fig 5E & 5F)

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We subsequently performed quantitative measurement of ONSD using standardized amplitude modulation (A-scan) echography with tissue sensitivity settings, placing the 8 MHz A-scan probe on the temporal eye equator in primary gaze position, as previously described by others [24, 25]