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Primary cultures of satellite cells were prepared from the upper hindlimb and back muscles of 910 month-old male rats and cultured for 24 h beginning at 24-h post-plating in DMEM-10% HS containing 5 ng/ml HGF that was pre-treated with GSSSG and LASSS separately at 1:4000 molar ratio just prior to peroxynitrite treatment (1:2000 to HGF

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Proper peptide storage and handling prove essential, with lyophilized peptides requiring storage at -20C or lower (optimally -80C) away from bright light and moisture, and peptides containing cysteine, methionine, or tryptophan requiring additional protection from oxidation through anaerobic storage conditions to maintain stability and biological activity throughout experimental protocols

Satarug S, Garrett SH, Sens MA, Sens DA