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To assess the half maximal inhibitory concentration (IC50) of FOXO4-DRI on the serum limitation-induced senescent fibroblast and the non-senescent control fibroblast, the fibroblast viability in the 2% FBS induced senescent fibroblast and 10% FBS cultured non-senescent control fibroblast with increasing concentrations of FOXO4-DRI was detected, and the cell viability curves were showed in Supplementary Fig

A GPx standard solution was prepared by dissolving 417 mg of GPx in 100 mL of 100 mM phosphate buffer at pH 7.0

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Real-time monitoring of glutathione in living cells reveals that high glutathione levels are required to maintain stem cell function