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Phosphate buffer (100 mM K 2 HPO 4 /KH 2 PO 4 , 1 mM EDTA, pH 7.4) with 1 M of the respective roGFP according to the information given in the text were pipetted into the wells of a 96-well plate with a clear bottom (NUNC 96, www.thermoscientific.com)
During the initial step, the thiol group of GSH is conjugated to the azo double bond of Ru(II)-OH, causing a reduction to a hydrazo moiety
Vortex (5 count)
J Plant Physiol 203:6983
R.KempurajD.MarchandJ
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