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MicroRNAs being small and potent regulators of gene expression, are infamous for their role in regulation of mRNA expression and aiding in post transcriptional modifications and their association with gynaecological disorders and impact on the endocrine system, typically the Hypothalamic-Pituitary-Ovarian axis has garnered an enormous amount of attention

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Esr1 -deficient mice ( B6N(Cg)-Esr1 tm4.2Ksk /J ) 53 were purchased from Jackson Laboratories and bred from heterozygous male and female mice