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Cells were passaged into a new 6-well plate at a density of 1.2 10 6 cells per well, ~30 min before transfection in 2 mL of 2i medium and transfected with 250 L of Opti-MEM containing 8 L of Lipofectamine 3000 and 46 g of plasmid DNA
Some figures have been created with the help of BioRender image and illustration software (BioRender.com)
IVs are based on a liter of fluid, which is equivalent to two gallons in the system
Can I combine this with IV Therapy or other treatments
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