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Researchers were looking for free glutathione levels, and because glutathione is so valuable, it is quickly bound to transport proteins to be carried to cells, causing it to not show up as free glutathione in the blood
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Briefly, in a 200 l reaction volume, 5 g ml 1 recombinant GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 mM DTT) was incubated with DMSO or different concentrations of inhibitors for 30 min at 37 C
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Why is vitamin B12 important
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