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elucidated that sulforaphane restored the downregulation of SLC7A11/GSH, suppressed ferroptosis, and improved cardiac function by activating the AMPK/Nrf2 pathway in the myocardium of type 2 diabetic mice [48]

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Immunocytochemistry and quantification of epSPC-neurospheres cultures Before the fixation, cells were washed with PBS for three times and then were fixed using 4% paraformaldehyde (Sigma) in PBS pH 7.4 for 15 min at room temperature (RT)