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Quantification of fluorescence signals Fluorescence intensity signals at actin patches or at the base of DNA bridges were quantified using the LCS Lite polygon tool by analyzing an image area of 40 m 2 around the base of the DNA bridge, and intensity values were normalized versus background values obtained by analyzing a nearby identical area within the cell, by subtracting the background-signal value from the actin-patch or the DNA bridge-base value (Dandoulaki et al, 2018)

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