So too was the Greek born scientist Stratis Avrameas and his colleagues at the Pasteur Institute in Villejuif, France, who also published a method for using the enzyme (Avrameas, Uriel)
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Example 22 preparation of Semetreuptade 60.5 g of the peptide resin obtained in example 18 was charged into a 2000ml single-neck flask, and lysate 600ml of TFA was prepared in advance: and (3) TIS: EDT (electro-thermal transfer coating): PhOH: h 2 Adding the lysate into a flask, reacting at room temperature for 2.5 hours, filtering off the resin, washing the resin with 50ml of TFA, combining filtrates, adding 6000ml of anhydrous ether to precipitate a white solid, centrifuging, washing the solid with the anhydrous ether, and drying in vacuum to obtain 20.56 g of crude semaglutide peptide as the white solid with the yield of 100.00 percent, wherein O is 90:3:3:2:2 (volume ratio)
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