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Cells were washed to remove viral particles and transduced cells were selected in the presence of 1 g/ml puromycin (Sigma-Aldrich, P8833) for at least 96 h

A useful way to frame the difference: tretinoin is a top-down instruction that reprograms the epidermis, whereas GHK-Cu is a bottom-up supply of signal and cofactor that helps the dermis rebuild

These include cysteine, glycine, and glutamic acid

Through glutathione S-transferase enzymes, making them water-soluble for excretion

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