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Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol
1:200) for 30 min
No air injection
Huang et al., 2015), stomach (Sikiric et al., 1997a
It boosts metabolism by helping the liver detoxify and metabolize fats, and reducing oxidative stress, which further improves insulin sensitivity and aids in weight management
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