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10.2337/dc18-2028 13 BikasA.JensenK.PatelA.CostelloJ.McDanielD.Klubo-GwiezdzinskaJ.et al (2015)

Lastly, the column goes through an elution step where target molecules are released from the ligands and collected, separately, as newly purified proteins

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Patients may feel brief warmth or tingling as energy is delivered, but discomfort is typically mild and temporary