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Having far more injections than your levels call for adds cost and appointments without a matching benefit, which is why frequency should track a confirmed need rather than a habit

At 72 h after the addition of doxycycline, cells were transferred to complete KSR media containing a final concentration of 0.5 mM l-Serine- 13 C 3 (Sigma-Aldrich, 604887)

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Frequency depends on your goals and the protocol