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Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose

DOI: Published online: November 8, 2021 1 Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea 2 Asan Diabetes Center, Asan Medical Center, Seoul, Korea 3 Department of Internal Medicine, H Plus Yangji Hospital, Seoul, Korea Corresponding author: Woo Je Lee Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea E-mail: [email protected] *Hwi Seung Kim and Taekwan Yoon contributed equally to this study as first authors

Colonies were screened under UV microscopy for basal expression of GFP (Figure 1c)

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