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VIP exerts its effects by binding to specific G-protein coupled receptors, notably VPAC1 and VPAC2 receptors, which are present in various tissues including the brain, gastrointestinal tract, and immune system (3)

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Knowledge about transporters expressed at the BBB and structural requirements for substrate binding and translocation via the transporters have been used for development of transporter-mediated drug delivery systems targeting the brain via specific transporter proteins

Next, to explore whether the X-IPA system could detect changes to gut permeability induced by intestinal metabolites, gut organ cultures were infused with putrescine, a polyamine compound produced by the gut microbiota which we recently identified as a potent disruptor of the intestinal epithelial barrier 10

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