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qPCR was performed with SYBR Green Supermix (TaKaRa) with the protocol as follow: 95 C for 5 min, repeating 40 cycles of 95 C for 5 s and 60 C for 45 s using StepOnePlus real-time PCR system (Applied Biosystem, Foster City, CA, USA)

Even though so many factors come into play, the average metabolic rate to remove alcohol is about one drink per hour

HPLC separates mixture components and produces chromatographic peaks that can be interpreted in relation to retention time, peak area, and separation quality [21] [25]

Probing human sperm metabolism using 13 C-magnetic resonance spectroscopy

For glutathione, this is a major hurdle