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Finally, the tissue sections were washed thoroughly with PBS, counterstained with DAPI and imaged under a laser scanning confocal microscope (FV-1000, Olympus, Japan)
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The gap between doing it right and doing it wrong comes down to three variables most guides never mention: injection angle into the vial, the specific type of water used, and ambient temperature during the entire procedure
[31] Thus, a deficiency in B 12 can cause accumulation of methyl-THF, mimicking folate deficiency
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